WebSep 27, 2024 · The Warburg Effect has emerged as a potential drug target because, in some cancer cell lines, it is sufficient to subvert it in order to kill cancer cells. It has also been shown that the Warburg Effect occurs in innate immune cells upon infection. Innate immune cells play critical roles in the tumour microenvironment but the Warburg Effect is not fully … WebApr 3, 2014 · THP1-Luciaâ ¢ ISG Cells - InvivoGen. EN. English Deutsch Français Español Português Italiano Român Nederlands Latina Dansk Svenska Norsk Magyar Bahasa …
Small molecule inhibition of human cGAS reduces total …
WebJul 5, 2024 · In the human fibroblast cell line BJ-5ta stably expressing a Halo-tagged cGAS, cGAS formed puncta with fluorescein-labeled ISD in the cytoplasm (Fig. 2A and fig. S4A). ... THP-1–Lucia ISG cells, which harbor a luciferase gene under the ISG54 promoter, ... WebRAW-Lucia™ ISG cells were generated from the murine RAW 264.7 macrophage cell line by stable integration of an interferon regulatory factor (IRF)-inducible Lucia luciferase … hobbit art cute stars in eyes
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WebMar 3, 2024 · To measure cGAMP levels using THP-1 Lucia ISG cells, 2.5×10 5 reporter cells were seeded in a 96-well plate in 50 μL serum-free DMEM supplemented with 50 ng/mL recombinant Perfringolysin O (PFO). 5 μL supernatant of heated lysate supernatant (see above) was added to each well for 16-hr at 37°C. 20 μL of this culture was transferred into … WebRAW-Lucia ISG cells and THP1-Dual ISG cells were used to determine IRF pathway activation by the indicated formulations. Cells (180 μL) were inoculated in 96 well plates (100,000 cells/well) with DMEM and RPMI-1640 medium, respectively, and incubated for 24 h at 37 °C in 5% CO 2 . WebTHP1-Lucia™ cells were specifically designed for monitoring the NF-κB signal transduction pathway in a physiologically relevant cell line. THP1-Lucia™ were derived from the … hrt block change