WebThe majority of the mutants (88%) segregate in a Mendelian manner for the mutant phenotype. An analysis of approximately 50 mutants in this group shows that > 80% are tagged with a functional insert. ... that it may be feasible to develop a comprehensive collection of mutant lines in which each gene is tagged by a T-DNA insertion. References WebTransfer DNA (T-DNA) insertion mutants are often used in forward and reverse genetics to reveal the molecular mechanisms of a particular biological process in plants. To generate …
TAIR - Mutant/Mapping Resources - Arabidopsis
http://jgi.doe.gov/wp-content/uploads/2015/05/T-DNA-genotyping.pdf WebThe CelR mutant protein bound DNA more weakly than the wild-type protein and formed a less stable complex with DNA in the presence of cellobiose. The results of Western analysis and gel retardation experiments suggest that CelR is produced constitutively and its DNA-binding activity is regulated through posttranslational modification. brahmin string
Comprehensive characterization of T-DNA integration induced
WebNov 7, 2006 · Abstract. Insertional mutagenesis approaches, especially by T-DNA, play important roles in gene function studies of the model plant Arabidopsis thaliana.GABI-Kat SimpleSearch (Author Webpage) is a Flanking Sequence Tag (FST)-based database for T-DNA insertion mutants generated by the GABI-Kat project.Currently, the database … WebT-DNA-tagged rice plants were screened under cold- or salt-stress conditions to determine the genes involved in the molecular mechanism for their abiotic-stress response. Line 0-165-65 was identified as a salt-responsive line. The gene responsible for this GUS-positive phenotype was revealed by inve … WebFigure 2 (A) Integrated genomics viewer highlighting the presence of a C>T at codon 790 (EGFR T790M) mutation (red) oriented in cis with a G>A at codon 796 (EGFR G796S) mutation (green).Overlapping reads spanning the T790M and G796S indicate cis orientation on the same allele.(B) The RTK sequence alignments across relevant TKIs … brahmin store in houston texas