Phospho-stat3 tyr705 cellular kit

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August undefined, 2024

WebThis Sandwich ELISA kit is an in vitro enzyme-linked immunosorbent assay for the measurement of phospho-STAT3 (Tyr705) and total STAT3 in human, mouse and rat cell lysates (help normalize the results of phospho-STAT3 from different cell lysates being compared). A pan STAT3 antibody has been coated onto a 96-well plate. WebPhosphorylation on tyrosine 705 by JAK1 and JAK2 is essential for STAT3 dimer formation, nuclear translocation, and DNA binding activity. In humans, the STAT3 gene is located on …

Phospho-STAT3 (Tyr705) Kit Meso Scale Discovery

WebAn anti-pan STAT3 antibody has been coated onto a 96-well plate. Samples are pipetted into the wells and STAT3 present in a sample is bound to the wells by the immobilized … WebThe MILLIPLEX® MAP STAT Phosphoprotein 5-plex Magnetic Bead Kit is used to detect changes in phosphorylated STAT1 (Tyr701), STAT2 (Tyr690), STAT3 (Tyr705), STAT5A/B (Tyr694/Tyr699), and STAT6 (Tyr641) in cell lysates using the Luminex®® system. The MILLIPLEX® MAP assay is a rapid, convenient alternative to Western fish paws severna park md

ab126459 – STAT3 (pY705) + total STAT3 ELISA Kit - Abcam

WebMembranes were probed with 0.5 µg/mL (1:1000 dilution) of purified anti-STAT3 Phospho (Tyr705) antibody (clone 13A3-1) for 2 hours at room temperature. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG antibody (Cat. No. 405306) at a 1:3000 dilution. Purified anti-STAT3 antibody (clone 4G4B45) was used as a ... WebOncostatin M (OSM) and leukemia inhibitory factor (LIF) signaling protects the heart after myocardial infarction (MI). In mice, oncostatin M receptor (OSMR) and leukemia inhibitory factor receptor (LIFR) are selectively activated by the respective cognate ligands while OSM activates both the OSMR and LIFR in humans, which prevents efficient translation of … WebCST's PathScan ® Phospho-Stat3 (Tyr705) Sandwich ELISA Kit is a solid phase sandwich enzyme-linked immunosorbent assay (ELISA) that detects endogenous levels of Phospho … fishpaws marketplace arnold md

XZH-5 inhibits STAT3 phosphorylation and causes apoptosis

MILLIPLEX MAP Phospho/Total STAT3 Magnetic Bead 2-Plex Kit - Cell …

WebThe Cell-Based Stat3 (Tyr705) ELISA kit is a very rapid, convenient, and sensitive assay kit that can monitor the activation or function of important biological pathways in ... Anti-Phospho-Stat3 (Tyr705) Anti-Stat3 EGF concentrations 0 20 100 ng/ml Fig. 3A. A431 cells were stimulated by different concentrations of EGF for 10 min at 37 o C m 0. ... WebStat3-Tyr705磷酸化位点抗体#9131R, 是JAK/STAT Signaling类化合 ... Anti-infection Apoptosis Autophagy Cell Cycle Cytoskeleton DNA Damage Endocrinology/Hormones … fishpaw tax service bucyrusWebMouse/Human/Rat Phospho-STAT3 (Tyr705) (Cell-Based Phosphorylation ELISA) ELISA Kit - LS-F1625. Submit Review LSBio Guarantee. Description: LS-F1625 is a 96-well enzyme … candice swanepoel freund

"WebMar 15, 2024 · Mouse monoclonal antibody recognizing O-GlcNAc (PTM-952) were purchased from PTM Biolabs Inc. Rabbit polyclonal antibodies recognizing STAT3 (AF1492), Phospho-STAT3 (Tyr705) (AF1276) were purchased from Beyotime Biotechnology. HRP Goat Anti-Rabbit IgG (AS014) was purchased from ABclonal Technology. " - Phospho-stat3 tyr705 cellular kit

Phospho-stat3 tyr705 cellular kit

STAT Phosphoprotein 5-Plex Magnetic Bead Kit 96-well Plate …

WebHere, we investigated the potential effects of IL-32α on epithelial–mesenchymal transition, metastasis, and invasion, and the JAK2/STAT3 signaling pathway in pancreatic cancer … Webphosphorylated at Tyr705. Upon activation, STAT3 is phosphorylated and after lysis of the cell membrane, phospho-STAT3 (Tyr705) can be detected using the kit reagents. FRET signal. Phospho-STAT3 Phospho-STAT3. Phospho-STAT3 Eu . Cryptate antibody. Phospho-STAT3 d2 antibody. Figure 1: Principle of HTRF sandwich assay. As shown …

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WebMouse/Human/Rat Phospho-STAT3 (Tyr705) (Cell-Based Phosphorylation ELISA) ELISA Kit - LS-F1625. LS-F1625 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the Qualitative detection of Human STAT3 in samples of Adherent Cell Cultures. It is based upon a Cell-Based Phosphorylation assay principle. WebSTAT3 (Phospho-Tyr705) Colorimetric Cell-Based ELISA Kit SKU: CBCAB00020 Product type: ELISA Kit ELISA Type: Cell Based Research Area: Immunology €599 Quantity: …

WebApr 12, 2024 · (A) The time dependence of FLU effect on STAT3 and phospho-STAT3 (Tyr705) expression. Cell were treated with 0.5 µM FLU and harvested in three time … WebMILLIPLEX MAP Phospho/Total STAT3 Magnetic Bead 2-Plex Kit - Cell Signaling Multiplex Assay Intracellular Bead-Based Multiplex Assays using the Luminex technology enables the simultaneous relative quantitation of multiple phosphorylation or total pathway proteins in tissue and cell lysate samples. The analytes available for this multiplex kit are: STAT3 …

WebJun 25, 2016 · XZH-5 inhibits STAT3 phosphorylation and causes apoptosis in human hepatocellular carcinoma cells ... According STAT3DNA binding ELISA Kit (Active Motif, Carlsbad, CA), cells were seeded 10cm plate. ... [31] STAT3dimerization inhibitor Stattic [32, 33] overnight. Fig.1a, treatmentdecreased STAT3 phosphorylation Tyr705 … WebSTAT3 can also be activated by Interferon-alpha, Interferon-gamma, EGF, PDGF and IL6. Phosphorylation on tyrosine 705 by JAK1 and JAK2 is essential for STAT3 dimer formation, nuclear translocation, and DNA binding activity. In humans, the STAT3 gene is located on the q arm of chromosome 17. STAT3 has been shown to be activated by IFN-alpha but ...

WebIt can be used for measuring the relative amount of Stat1 (pTyr 701), Stat3 (pTyr 705) and Stat5 (pTyr 694) phosphorylation and screening the effect of various treatment, inhibitors (such as siRNA or chemicals), or activators in cultured human, mouse and rat cell lines (Stat5 only can be used for detecting human cell lines). 免疫原

WebKit-Specificity Information This assay kit contains antibodies which recognize the phospho-Tyr705-epitope, and a distal epitope on STAT3. α. and STAT3β. The proteins detected by this kit correspond to GenBank Accessions NP_644805 and NP_998827. These antibodies recognize STAT3 of human, mouse and rat origin. Other species should be tested on ... candice swanepoel fashionWebThe Total STAT3 cellular assay kit is designed to monitor the expression level of STAT3, whether phosphorylated or unphosphorylated. It is compatible with our Phospho-STAT3 kit, and enables the analysis of phosphorylated and total proteins from a single sample for better readout of the JAK/STAT signaling pathway. candice swanepoel first vs fashion showWebTumor heterogeneity dominates tumor biological behavior and shapes the tumor microenvironment. However, the mechanisms of tumor genetic features modul… fish pay ponds near meWebDec 1, 2024 · E STAT3 mutants were overexpressed in HM20 and CL1-5 STAT3-KO [an sgRNA was designed to target human STAT3, and STAT3 knockout was performed by CRISPR/Cas9 technology] cells prior to incubation ... candice swanepoel hdWebSTAT3 is activated by Tyr705 phosphorylation in response to membrane factors such as interleukin-6 (IL-6). 45 It has also been shown that Ser727 of STAT3 is phosphorylated by various kinds of kinases in various cancers. 46–49 However, some reports have shown that the Ser727 site phosphorylation suppresses STAT3 activity and cell proliferation ... fishpaw tax service bucyrus ohioWebFeb 2, 2008 · Since EGFRvIII associated efficiently with STAT3 but failed to significantly alter STAT3 phosphorylation at Tyr705 or Ser727, we further characterized the interaction of … candice swanepoel hair commercialWebA Life Sciences and Diagnostics business focused on enabling scientists and healthcare professionals to advance research, discovery and detection to improve global health … fish paykel dishwasher pump dw60cew1