Phospho-akt1/2/3 ser473 cellular kit

WebThe protocol is simple and straightforward, with just two incubation steps: - Cell stimulation by the target compounds - cAMP detection using HTRF reagents Product specifications Designed with signal performance in mind, the kit offers a tailored standard curve ideally suited to the study of Gi coupled GPCRs. Introduction to product validation Web#IR172-668 / IR172-668,Phospho-AKT (Ser473) antibody. Products ... and integrin-linked kinase. These AKT proteins therefore regulate a wide variety of cellular functions including cell proliferation, survival, metabolism, and angiogenesis in both normal and malignant cells. ... Subsequent phosphorylation of both threonine residue 308 and serine ...

Phospho-AKT1 (Ser473) Monoclonal Antibody (SDRNR), PE (12 …

LANCE Ultra phospho-AKT1/2/3 (Ser473) kits are designed for the detection of AKT1, AKT2, and AKT3 (phosphorylated at residue S473) in cell lysates using a simple, homogeneous LANCE Ultra assay (no wash steps). This assay is compatible with both adherent and suspension cells. WebDec 16, 2009 · PIP3 recruits protein dependent kinase 1 (PDK1) and AKT, also known as protein kinase B (PKB), to the plasma membrane where AKT is phosphorylated at Thr308 by PDK1 and at Ser473 by mTOR complex 2 (TORC2) . AKT1 can also be phosphorylated at Ser473 by DNA-PK in the nucleus . AKT occupies a key regulatory node in the PI3K … fit and you know it https://mrhaccounts.com

Phospho-AKT1 (Ser473) Recombinant Rabbit Monoclonal …

WebApr 12, 2024 · Sex related differences have been observed in the progression of Alzheimer’s disease (AD) [1] and the prevalence of AD is greater in women than men in European and American patients [2, 3 ... WebProduct Note This antibody is raised against human AKT1 phosphorylated at Ser473. Based on sequence homology, it is predicted to react with AKT2 and AKT3 when phosphorylated at the corresponding residues - AKT2 Ser474 and AKT3 Ser472. Predict Reactivity Zebrafish, Sheep, Bovine, Cat, Chicken, Rhesus Monkey (>80% identity) PROPERTIES TARGET WebThe phospho-EGFR assay kit is designed for the cell-based quantitative detection of Tyr1068 phosphorylation on EGFR. Mutations that lead to an overexpression or hyperactivity of EGFR, also known as ErbB1 or HER1, are associated with a number of cancers, such as lung, colorectal, prostate, glioblastoma cancer, etc. can ferrothorn learn toxic spikes

Serine/threonine-protein kinase 24 is an inhibitor of gastric cancer ...

Category:Phospho-AKT 1/2/3 (Ser473) LANCE Ultra TR-FRET …

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Phospho-akt1/2/3 ser473 cellular kit

Drug-screening and genomic analyses of HER2-positive breast …

WebPhospho-AKT1 (Ser473) Antibody (700392) in ICC/IF Immunofluorescent analysis of AKT (pS473) was done on 70% confluent log phase U-87 MG cells. The cells were fixed with 4% paraformaldehyde for 15 minutes; permeabilized with 0.25% Triton X-100 for 10 minutes followed by blocking with 5% BSA for 1 hour at room temperature. WebThe Advanced Phospho-ERK assay kits are designed for the robust and highly sensitive quantification of ERK phosphorylation on Thr202/Tyr204 as an MAPK pathway readout. The simple mix-and-read protocol eliminates all wash steps for faster analysis and high quality output. The Total-ERK1/2 assay is compatible with the buffers from the Phospho or ...

Phospho-akt1/2/3 ser473 cellular kit

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WebAKT is a serine/threonine protein kinase that plays a key role in multiple cellular processes including metabolism, proliferation, apoptosis/survival, and migration. There are three homologous isoforms of AKT: AKT1, AKT2, and AKT3. AKT is activated by binding of its pleckstrin homology (PH) domain to membrane phospholipids and by phosphorylation. WebSep 15, 2024 · The protein concentration of the supernatants was measured by BCS TM protein assay kit according to manufacturer’s ... The knockout of STK24 expression did …

WebFeb 3, 2024 · Protein kinase B (AKT1) is hyper-activated in diverse human tumors. AKT1 is activated by phosphorylation at two key regulatory sites, Thr308 and Ser473. Active AKT1 phosphorylates many, perhaps hundreds, of downstream cellular targets in the cytosol and nucleus. AKT1 is well-known for phosphorylatin … WebBath Filler and Heat Transfer Fluids; Bath Specific Platforms and Clamps; Bath Specific Tube Racks; Calibration Baths; Circulating Baths; Coliform Baths

Web寻找 Abcam 抗体及试剂 - Abcam中文官网 WebThe Phospho-AKT3 (Ser473) kit is designed for the robust quantification of AKT3 modulation, phosphorylated on Ser473. Also known as protein kinase B (PKB), AKT is an …

WebPhospho-Akt (Ser473) Kit For use with human, mouse, rat, and non-human primate cell lysates and tissue lysates. This package insert must be read in its entirety before using …

WebNov 25, 2015 · The ER also plays a critical role toward maintenance of cellular calcium homeostasis. ... and the following oligonucleotide primers were used for RT-PCR: Akt1 forward, 5′-CTGTCTCGAGAGCGTGTGTT-3′; Akt1 reverse, 5′-AACAGCTTCTCGTGGTCCTG-3′; Akt2 ... [rabbit polyclonal anti-phospho-Akt (Ser473) antibody (Cell Signaling Technology, … can fertile chicken eggs be refrigeratedWebPhospho-Akt (Ser473) Antibody detects endogenous levels of Akt1 only when phosphorylated at Ser473. This antibody also recognizes Akt2 and Akt3 when … canfestival transmission typeWebphospho-AKT 1/2/3 (Ser473) Detection Antibody Reagent Positive Control Cell Lysate (lyophilized) Detection Reagent (TMB Substrate) Stop Solution Plate seal Description … canfestival timer_max_countWebThe phospho-AKT1 (Ser473) kit is designed for robust quantification of AKT1 modulation, phosphorylated on Ser473. Also known as protein kinase B (PKB), Akt is an oncogene … fit and you know it the streetsWebAKT, also known as protein kinase B, is an important regulator of numerous cellular processes including cell survival, glucose metabolism, transcription, and apoptosis. AKT … canfestival stm32f103WebAkt1/2 kinase inhibitor indeed decreased pAkt in all four cell lines , while total Akt levels were not affected by the treatment. To determine whether induction of apoptosis caused … fit and youWebThe Phospho-AKT (Ser473) kit is designed to robustly and efficiently monitor AKT phosphorylation on Ser473 across all isoforms, as a readout of Pi3K pathway activation. … can fertilizer be applied to wet grass