WebThe complicity of human RAS proteins in cancer is a well-documented fact, both due to the mutational hyperactivation of these GTPases and the overexpression of the genes encoding these proteins. Thus, it can be easily assumed that the study of RAS genes at the transcriptional and post-transcriptional level is of the utmost importance. Although … Web21 sep. 2024 · From a genetic point of view, LGMD2A is caused by mutations located within the Calpain 3 (CAPN3) gene ... The template amplification and enrichment steps were …
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WebMassively parallel sequencing was carried out on a Personal Genome Machine (PGM) sequencer (Ion Torrent™) using the Ion PGM™ Hi-Q™ Sequencing Kit or Ion S5 sequencer (Thermo Fisher Scientific) using Ion S5 Sequencing kit according to the manufacturer’s instructions. Sequencing was performed using 500 flow runs that … Web21 sep. 2024 · From a genetic point of view, LGMD2A is caused by mutations located within the Calpain 3 (CAPN3) gene ... The template amplification and enrichment steps were performed by an Ion PGM Hi-Q OT2 kit-400, Ion OneTouch 2 System and an Ion OneTouch ES (Thermo Fisher Scientific, Foster City, CA, USA). Web7 apr. 2024 · NGS was performed using an Ion Plus Fragment Library Kit, a Hi-Q View OT2 Kit, a Hi-Q View Sequencing Kit, or an Ion 318 Chip Kit v2 (Thermo Fisher Scientific) … crypto credit card singapore