WebApplication CTLL-2 has been used in CTLL-2 cell proliferation assay to determine the presence of T-cell cytokines. [ 1] [ 2] Culture Medium RPMI 1640 + 2mM Glutamine + 10-20 IU/ml Human Recombinant IL-2 + 10% Foetal Bovine Serum (FBS). Subculture Routine Maintain cultures between 3-9 x 10 5 cells/ml; 5% CO 2; 37°C. Webfrom the Mo7e and CTLL-2 cyto-kine-dependent proliferation assay, PFC-1 exhibited an approx-imately 10-fold stronger potency than rhIL-15 in PBMC prolifera-tion, while 10 nM rhIL-15 led to a proliferation rate of 20.71% and 1 nM PFC-1 resulted in a mean value of 22.05% (Fig. 2B). The enhanced activity could be due to the higher activity of IL ...

CTLL-2 Cell Line - an overview ScienceDirect Topics

WebCTLL-2 proliferation assay CTLL-2 cells were washed twice with prewarmed HBSS (Gibco, # 14175095) and then grown in the absence of IL-2 for 24 h in RPMI1640 (Gibco, … WebNov 29, 2013 · Most bioassays measured the proliferative effect of IL-2 on the murine cytotoxic T cell-line, CTLL-2 or the HT-2 cell-line but employed different readouts for assessing the proliferation (Gillis et al., 1978, Gieni et al., 1995). In one laboratory, however, the human T cell-line, KIT 225 was used (Hori et al., 1987). dynamics 365 iom trial

Interleukin-2 Carbohydrate Recognition Modulates CTLL …

WebCTLL-2 is a clone of cytotoxic T cells derived from a C57BL/6 mouse. The cells are dependent upon Interleukin-2 (IL-2) for growth and can be used to assay for IL-2. … WebProliferation: IL-2 : CTLL-2 (ATCC TIB-214) 2: 24: 10: ≤175pg/mL: Proliferation: IL-3 : TF-1 (ATCC CRL-2003) 2: 48: 10: ≤0.6-0.15ng/mL: Proliferation: IL-4 : TF-1 (ATCC CRL-2003) 2: 48: 10: ... Cytotoxicity Assay: VEGF 121: HUVEC: 2: 72: 1000: ≤8-10ng/mL: Proliferation: Mouse Cytokine Bioassay Quick Guide Cytokine Indicator Cells Cell ... WebTitle: CTLL-2 Cell Proliferation Assay for Determination of Bioactivity of IL-15 . This procedure is made available through federal funds from the National Cancer Institute, NIH, under contract . UNCONTROLLED COPY FOR TRAINING AND REFERENCE PURPOSES ONLY . 7.5.2 Using a 50 -300 multiple channel pipettes, add the Starting Std01 or sample dynamics 365 invoice processing